Genre
- Journal Article
This study demonstrates a novel link between alterations in platelet-derived growth factor (PDGF) regulation of ornithine decarboxylase (ODC) expression during malignant conversion. H-ras-transformed cell lines exhibited PDGF-mediated alterations in ODC gene expression. These alterations involved transcriptional, posttranscriptional, and cycloheximide-mediated events. PDGF-mediated alterations in ODC gene expression in NR3 cells (capable of only benign tumour formation) were ras-dependent, involved a tyrosine kinase activity and mitogen-activated protein (MAP) kinase-mediated signalling events, and were independent of both protein kinase C (PKC) events and pertussis toxin-sensitive (PTS) G-protein-mediated signalling. PDGF-mediated alterations in ODC gene expression in C2 cells [capable of malignant progression (metastasis formation)] were ras-dependent, required a tyrosine kinase activity, involved both MAP kinase-mediated events and phosphatidylinositol-3-kinase (PI-3-kinase)-mediated events, and were dependent upon PTS G-protein-mediated signalling but independent of PKC-mediated events. PDGF-mediated regulation of ODC gene expression changes in response to H-ras-mediated cellular transformation and malignant progression. (C) 2001 Elsevier Science Inc. All rights reserved.
Univ Toronto, St Michaels Hosp, Dept Lab Med & Pathobiol, Toronto, ON, Canada.; Hurta, RAR, Univ Toronto, St Michaels Hosp, Dept Lab Med & Pathobiol, 1-001,E Annex,38 Shuter St, Toronto, ON, Canada.
NEW YORK; 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA
ELSEVIER SCIENCE INC
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Source type: Electronic(1)
Language
- English
Subjects
- MALIGNANT TRANSFORMATION
- FACTOR-BETA(1)
- POLYAMINES
- PDGF
- Differentiation
- FIBROBLASTS
- ornithine decarboxylase
- NIH 3T3 CELLS
- cellular transformation
- Cell Biology
- MESSENGER-RNA
- PROTEIN-KINASE-C
- INHIBITOR
- GENE-EXPRESSION