Microsatellite markers from a microdissected swine chromosome 6 genomic library

A microdissected pig genomic library was generated from the proximal half of the q arm of chromosome 6. Purified DNA was digested with restriction enzymes, ligated to oligonucleotide adaptors and amplified by PCR using primers complementary to the adaptor sequences. The purity of the amplified products and boundaries of the microdissected chromosomal region were verified by fluorescence in situ hybridization. (CA)n-containing sequences were identified in a small insert genomic library generated from the PCR-amplified microdissected DNA. Oligonucleotide primers were developed for PCR amplification of 30 of the 46 (CA)n repeat-containing clones, and were subsequently used to amplify DNA isolated from unrelated pigs of different breeds to determine the informativeness of these microsatellite markers. 22 these markers were mapped using the University of Illinois Yorkshire [Large White] x Meishan reference population. These 22 markers were all assigned to a 50.7-cM region of the pig chromosome 6 linkage map..