Genre
- Journal Article
Determining the accuracy and precision of a measuring instrument is pertinent in antimicrobial susceptibility testing. This study was conducted to predict the diagnostic accuracy of the Sensititre MIC mastitis panel (Sensititre) and agar disk diffusion (ADD) method with reference to the manual broth microdilution test method for antimicrobial resistance profiling of Escherichia coli (n = 156), Staphylococcus aureus (n = 154), streptococcal (n = 116), and enterococcal (n = 31) bovine clinical mastitis isolates. The activities of ampicillin, ceftiofur, cephalothin, erythromycin, oxacillin, penicillin, the penicillin-novobiocin combination, pirlimycin, and tetracycline were tested against the isolates. Diagnostic accuracy was determined by estimating the area under the receiver operating characteristic curve; intertest essential and categorical agreements were determined as well. Sensititre and the ADD method demonstrated moderate to highly accurate (71 to 99%) and moderate to perfect (71 to 100%) predictive accuracies for 74 and 76% of the isolate-antimicrobial MIC combinations, respectively. However, the diagnostic accuracy was low for S. aureus-ceftiofur/oxacillin combinations and other streptococcus-ampicillin combinations by either testing method. Essential agreement between Sensititre automatic MIC readings and MIC readings obtained by the broth microdilution test method was 87%. Essential agreement between Sensititre automatic and manual MIC reading methods was 97%. Furthermore, the ADD test method and Sensititre MIC method exhibited 92 and 91% categorical agreement (sensitive, intermediate, resistant) of results, respectively, compared with the reference method. However, both methods demonstrated lower agreement for E. coli-ampicillin/cephalothin combinations than for Gram-positive isolates. In conclusion, the Sensititre and ADD methods had moderate to high diagnostic accuracy and very good essential and categorical agreement for most udder pathogen-antimicrobial combinations and can be readily employed in veterinary diagnostic laboratories.
Department of Production Animal Health, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada. vsaini@ucalgary.ca
United States
American Society for Microbiology : Washington
Accession Number: 21270215. Language: English. Language Code: eng. Date Revised: 20120427. Date Created: 20110405. Date Completed: 20110718. Update Code: 20120427. Publication Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't. Journal ID: 7505564. Publication Model: Print-Electronic. Cited Medium: Internet. NLM ISO Abbr: J. Clin. Microbiol. PubMed Central ID: PMC3122827. Cites: J Dairy Sci. 2008 Apr;91(4):1366-77. (PMID: 18349229). Cites: J Clin Microbiol. 1991 Jun;29(6):1249-51. (PMID: 1864944). Cites: J Vet Diagn Invest. 1991 Jan;3(1):66-71. (PMID: 2039791). Cites: J Dairy Sci. 1988 Jul;71(7):1934-9. (PMID: 3261743). Cites: Science. 1988 Jun 3;240(4857):1285-93. (PMID: 3287615). Cites: Diagn Microbiol Infect Dis. 1985 May;3(3):185-91. (PMID: 3922668). Cites: Am J Clin Pathol. 1966 Apr;45(4):493-6. (PMID: 5325707). Cites: Am J Clin Pathol. 1970 Jun;53(6):880-5. (PMID: 5504607). Cites: Vet Microbiol. 1982 Nov;7(5):447-54. (PMID: 6761955). Cites: J Clin Microbiol. 1983 May;17(5):878-86. (PMID: 6863508). Cites: Med Instrum. 1978 May-Jun;12(3):167-70. (PMID: 713903). Cites: J Clin Microbiol. 1980 Apr;11(4):426-9. (PMID: 7372803). Cites: Antimicrob Agents Chemother. 1980 Mar;17(3):464-9. (PMID: 7425606). Cites: Clin Microbiol Rev. 1994 Jul;7(3):346-56. (PMID: 7923054). Cites: Am J Vet Res. 1977 Oct;38(10):1503-7. (PMID: 931132). Cites: J Vet Diagn Invest. 1998 Apr;10(2):164-8. (PMID: 9576344). Cites: Prev Vet Med. 2000 May 30;45(1-2):23-41. (PMID: 10802332). Cites: J Vet Med B Infect Dis Vet Public Health. 2001 Feb;48(1):21-9. (PMID: 11254096). Cites: Microb Drug Resist. 2005 Winter;11(4):342-50. (PMID: 16359193). Cites: J Dairy Sci. 2006 Jun;89(6):1877-95. (PMID: 16702252). Cites: Lancet. 1986 Feb 8;1(8476):307-10. (PMID: 2868172). Cites: J Food Prot. 1998 Jun;61(6):742-56. (PMID: 9709262). Linking ISSN: 00951137. Subset: IM. Date of Electronic Publication: 2011 Jan 26; ID: 21270215
Language
- English
Subjects
- animals
- Anti-Bacterial Agents/*pharmacology
- cattle
- Bacteria/*drug effects
- Bacteria/isolation & purification
- Mastitis, Bovine/*microbiology
- Female
- Drug Resistance, Bacterial*
- Microbial Sensitivity Tests/methods