Zhang, Qijing, et al. “Identification of an Arsenic Resistance and Arsenic-Sensing System in Campylobacter Jejuni”. Applied and Environmental Microbiology, vol. 75, no. 15, 2009, pp. 5064-73, https://scholar2.islandarchives.ca/islandora/object/ir%3A1848.

Genre

  • Journal Article
Contributors
Author: Zhang, Qijing
Author: Sahin, Orhan
Author: Wang, Liping
Author: Jeon, Byeonghwa
Date Issued
2009
Abstract

Arsenic is commonly present in the natural environment and is also used as a feed additive for animal production. Poultry is a major reservoir for Campylobacter jejuni, a major food-borne human pathogen causing gastroenteritis. It has been shown that Campylobacter isolates from poultry are highly resistant to arsenic compounds, but the molecular mechanisms responsible for the resistance have not been determined, and it is unclear if the acquired arsenic resistance affects the susceptibility of Campylobacter spp. to other antimicrobials. In this study, we identified a four-gene operon that contributes to arsenic resistance in Campylobacter. This operon encodes a putative membrane permease (ArsP), a transcriptional repressor (ArsR), an arsenate reductase (ArsC), and an efflux protein (Acr3). PCR analysis of various clinical C. jejuni isolates indicated a significant association of this operon with elevated resistance to arsenite and arsenate. Gene-specific mutagenesis confirmed the role of the ars operon in conferring arsenic resistance. It was further shown that this operon is subject to regulation by ArsR, which directly binds to the ars promoter and inhibits the transcription of the operon. Arsenite inhibits the binding of ArsR to the ars promoter DNA and induces the expression of the ars genes. Mutation of the ars genes did not affect the susceptibility of C. jejuni to commonly used antibiotics. These results identify the ars operon as an important mechanism for arsenic resistance and sensing in Campylobacter.

Note

Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, 50011, USA.

United States

American Society for Microbiology : Washington

Accession Number: 19502436. Language: English. Language Code: eng. Date Revised: 20100927. Date Created: 20090727. Date Completed: 20090921. Update Code: 20111122. Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't. Journal ID: 7605801. Publication Model: Print-Electronic. Cited Medium: Internet. NLM ISO Abbr: Appl. Environ. Microbiol. PubMed Central ID: PMC2725487. Cites: Infect Immun. 2007 Jul;75(7):3425-33. (PMID: 17438034). Cites: Int J Food Microbiol. 2007 Jul 15;117(3):237-57. (PMID: 17368847). Cites: J Bacteriol. 2007 Oct;189(20):7399-407. (PMID: 17693521). Cites: J Bacteriol. 2007 Nov;189(22):8402-3. (PMID: 17873037). Cites: J Bioenerg Biomembr. 2007 Dec;39(5-6):453-8. (PMID: 17955352). Cites: J Bacteriol. 2008 Mar;190(6):1879-90. (PMID: 18178742). Cites: Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9474-8. (PMID: 1409657). Cites: Biochemistry. 1989 Mar 7;28(5):2331-9. (PMID: 2719955). Cites: J Bacteriol. 1995 Apr;177(8):2050-6. (PMID: 7721697). Cites: J Biol Chem. 1994 Aug 5;269(31):19826-9. (PMID: 8051064). Cites: J Biol Chem. 1993 Jan 5;268(1):52-8. (PMID: 8416957). Cites: J Biol Chem. 1996 Apr 19;271(16):9291-7. (PMID: 8621591). Cites: Appl Environ Microbiol. 1998 Feb;64(2):411-8. (PMID: 9464374). Cites: J Biol Chem. 1998 Jul 17;273(29):18665-73. (PMID: 9660841). Cites: Trends Microbiol. 1999 May;7(5):207-12. (PMID: 10354596). Cites: Mol Microbiol. 2001 Apr;40(1):214-24. (PMID: 11298288). Cites: Poult Sci. 2002 Mar;81(3):356-64. (PMID: 11902412). Cites: FEBS Lett. 2002 Oct 2;529(1):86-92. (PMID: 12354618). Cites: Mol Microbiol. 1993 May;8(3):615-23. (PMID: 8326869). Cites: J Biol Chem. 1996 Feb 2;271(5):2427-32. (PMID: 8576202). Cites: J Bacteriol. 1997 Feb;179(3):612-9. (PMID: 9006011). Cites: J Bacteriol. 1998 Feb;180(3):594-9. (PMID: 9457862). Cites: J Bacteriol. 1998 Apr;180(7):1655-61. (PMID: 9537360). Cites: Microbiology. 1998 Oct;144 ( Pt 10):2705-13. (PMID: 9802012). Cites: Nature. 2000 Feb 10;403(6770):665-8. (PMID: 10688204). Cites: Appl Environ Microbiol. 2000 May;66(5):1826-33. (PMID: 10788346). Cites: Biochemistry. 2001 Nov 6;40(44):13378-89. (PMID: 11683648). Cites: Microbiology. 2002 Apr;148(Pt 4):1203-12. (PMID: 11932464). Cites: Antimicrob Agents Chemother. 2002 Jul;46(7):2124-31. (PMID: 12069964). Cites: Microbiology. 2002 Dec;148(Pt 12):3983-92. (PMID: 12480902). Cites: J Mol Biol. 2003 Mar 7;326(5):1361-72. (PMID: 12595250). Cites: Science. 2003 May 9;300(5621):939-44. (PMID: 12738852). Cites: J Bacteriol. 2003 Sep;185(18):5363-71. (PMID: 12949088). Cites: J Biol Chem. 2004 Apr 23;279(17):17810-8. (PMID: 14960585). Cites: PLoS Biol. 2005 Jan;3(1):e15. (PMID: 15660156). Cites: Trends Microbiol. 2005 Feb;13(2):45-9. (PMID: 15680760). Cites: Mol Microbiol. 1991 Jun;5(6):1331-6. (PMID: 1838573). Cites: Gene. 1990 Sep 28;94(1):23-8. (PMID: 2227449). Cites: J Bacteriol. 1990 Feb;172(2):949-55. (PMID: 2404960). Cites: Nucleic Acids Res. 1995 Jul 11;23(13):2472-8. (PMID: 7630724). Cites: J Bacteriol. 1995 Jan;177(2):385-9. (PMID: 7814328). Cites: Mol Gen Genet. 1994 Mar;242(5):566-72. (PMID: 8121414). Cites: Antimicrob Agents Chemother. 2005 Mar;49(3):1067-75. (PMID: 15728904). Cites: Vet Res. 2005 May-Jun;36(3):351-82. (PMID: 15845230). Cites: J Bacteriol. 2005 Oct;187(20):6991-7. (PMID: 16199569). Cites: J Bacteriol. 2005 Nov;187(21):7417-24. (PMID: 16237025). Cites: Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2075-80. (PMID: 16452170). Cites: Appl Environ Microbiol. 2006 Apr;72(4):3069-71. (PMID: 16598022). Cites: Appl Environ Microbiol. 2006 May;72(5):3738-42. (PMID: 16672525). Cites: Microbes Infect. 2006 Jun;8(7):1967-71. (PMID: 16713726). Cites: Environ Sci Technol. 2006 May 1;40(9):2951-7. (PMID: 16719096). Cites: J Antimicrob Chemother. 2006 Aug;58(2):243-55. (PMID: 16735431). Cites: Infect Immun. 2006 Aug;74(8):4694-707. (PMID: 16861657). Cites: Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15617-22. (PMID: 17030823). Cites: Res Microbiol. 2007 Mar;158(2):128-37. (PMID: 17258434). Cites: Clin Infect Dis. 2007 Mar 1;44(5):701-3. (PMID: 17278063). Cites: Environ Sci Technol. 2007 Feb 1;41(3):818-23. (PMID: 17328188). Cites: J Bacteriol. 2007 May;189(10):3705-11. (PMID: 17337573). Cites: FEBS Lett. 2007 Aug 21;581(21):3996-4000. (PMID: 17673204). Linking ISSN: 00992240. Subset: IM. Date of Electronic Publication: 2009 Jun 05; ID: 19502436

Source type: Electronic(1)

http://search.ebscohost.com/login.aspx?direct=true&db=cmedm&AN=19502436&site=eds-live

Language

  • English

Subjects

  • Polymerase Chain Reaction/methods
  • Campylobacter jejuni/*drug effects
  • DNA, Bacterial/genetics
  • Arsenic/*pharmacology
  • Sequence Analysis, DNA
  • Membrane Transport Proteins/genetics
  • Operon
  • Poultry/microbiology
  • Gene Order
  • Repressor Proteins/genetics
  • Gene Expression Regulation, Bacterial
  • animals
  • Anti-Bacterial Agents/*pharmacology
  • Arsenate Reductases/genetics
  • Bacterial Proteins/genetics
  • Genes, Bacterial
  • DNA, Bacterial/chemistry
  • Microbial Sensitivity Tests
  • Drug Resistance, Bacterial*
  • Molecular Sequence Data
  • Campylobacter jejuni/isolation & purification
  • Electrophoretic Mobility Shift Assay
  • Base Sequence
  • Gene Knockout Techniques
Page range
5064-5073
Host Title
Applied and Environmental Microbiology
Host Abbreviated Title
Appl.Environ.Microbiol.
Volume
75
Issue
15
ISSN
1098-5336

Department