Kerr, R. G., and K. Kelly. “An Enzyme-Based Formaldehyde Assay and Its Utility in a Sponge Sterol Biosynthetic Pathway”. Journal of Natural Products, vol. 62, no. 1, 1999, pp. 201-2, https://doi.org/10.1021/np980366d.

Genre

  • Journal Article
Contributors
Author: Kerr, R. G.
Author: Kelly, K.
Date Issued
1999
Abstract

An enzyme-based assay has been developed and utilized to confirm the production of formaldehyde in the dealkylation of sterol side chains in a marine sponge. The enzyme used in the assay, formaldehyde dehydrogenase, is NAD+ dependent, and the assay measures the production of NADH by determining the increase in fluorescence at 460 nm.

Note

Department of Chemistry and Biochemistry, Center for Molecular Biology and Biotechnology, Florida Atlantic University, Boca Raton, Florida 33431-0991, USA.

Language

  • English
Page range
201-202
Host Title
Journal of Natural Products
Host Abbreviated Title
J.Nat.Prod.
Volume
62
Issue
1
ISSN
1520-6025
0163-3864
DOI
https://doi.org/10.1021/np980366d

Department