Genre
- Journal Article
A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 10(0.25) mean tissue culture infective dose (TCID50) and 10(-0.44) TCID50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.
ID: IND22076815; Accession Number: IND22076815 Pagination: p. 681-685. Identifiers: diagnostic value Publicaton Type: Journal Article Language(s): English Language Summary: Spanish Language Note: Summary in Spanish Bibliography Statement: Includes references Coden: AVDIAI Category Codes: Animal Diseases (Viral) Subset: Indexing Branch - NAL NAL Call Number: 41.8 Av5 Update Code: 00000000
Source type: Electronic(1)
http://search.ebscohost.com/login.aspx?direct=true&db=agr&AN=IND22076815&site=eds-live
Language
- English
Subjects
- reverse transcription
- detection
- accuracy
- polymerase chain reaction
- Pneumovirus