Genre
- Journal Article
In the first part of this study, generation times relative to temperature, together with cardinal and conceptual temperatures, were determined for four strains of Xenorhabdus bacteria that represented three geographically distinct species. The data showed that the NF strain of Xenorhabdus bovienii, like the Umea strain of the same species, is psychrotrophic, while Xenorhabdus sp. TX strain resembles Xenorhabdus nematophila All strain in being mesophilic. In the second part, the capacity of these bacteria to adapt to changes in temperature, shown by changes in fatty acid composition, was investigated. As temperature declined, the proportions of the two major unsaturated fatty acids, palmitoleic (16:1omega7) acid and oleic (18:1 pi9) acid, increased significantly in all of the strains. The proportion of the prevalent saturated fatty acid, which was palmitic acid (16:0), decreased. In the All, NF, and Umea strains, myristic acid (14:0), margaric acid (17:0), cyclopropane (17:0c), and arachidic acid (20:0) decreased with decreasing temperature. In the third part of the study, the synthesis of isozymes in response to changing temperature was investigated. For the seven enzymes studied, the numbers for which isozyme synthesis was temperature related were as follows: five for Umea, four for All, three for NF, and two for TX. Where the study dealt with fatty acid composition and isozyme synthesis, the results show a broad capacity for physiological temperature adaptation among strains of different climatic origin.
Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53201 USA. Univ Prince Edward Isl, Dept Biol, Charlottetown, PE C1A 4P3, Canada. Mem Univ Newfoundland, Dept Biol, St Johns, NF A1B 3X9, Canada.; He, HJ, Univ Wisconsin, Dept Biol Sci, POB 413, Milwau(TRUNCATED)
OTTAWA; RESEARCH JOURNALS, MONTREAL RD, OTTAWA, ONTARIO K1A 0R6, CANADA
NATL RESEARCH COUNCIL CANADA
PT: J; CR: AKHURST RJ, 1980, J GEN MICROBIOL, V121, P303 AKHURST RJ, 1982, J GEN MICROBIOL, V128, P3061 AKHURST RJ, 1990, ENTOMOPATHOGENIC NEM, P75 BLIGH EG, 1959, CAN J BIOCH PHYSL, V37, P911 BOEMARE N, 1997, SYMBIOSIS, V22, P21 BOEMARE NE, 1988, J GEN MICROBIOL 3, V134, P751 DUNPHY GB, 1990, ENTOMOPATHOGENIC NEM, P301 FISCHERLESAUX M, 1999, FEMS MICROBIOL ECOL, V29, P149 FISCHERLESAUX M, 1999, INT J SYST BACTERI 4, V49, P1645 FODOR E, 1997, APPL ENVIRON MICROB, V63, P2826 FORST S, 1996, MICROBIOL REV, V60, P21 GERHARDT P, 1994, METHODS GEN MOL BACT GOW JA, 1984, APPL ENVIRON MICROB, V47, P213 GREWAL PS, 1994, J THERM BIOL, V19, P245 GWYNN RL, 1994, IOBC WPRS WORKING GR, V17, P120 HATAB MAA, 1997, J APPL MICROBIOL, V82, P351 HE HJ, 2000, CAN J MICROBIOL, V46, P618 HEBERT PDN, 1989, METHODOLOGIES ALLOZY JAGDALE GB, 1996, J NEMATOL, V28, P301 JAGDALE GB, 1997, CAN J ZOOL, V75, P2137 JAGDALE GB, 1997, COMP BIOCHEM PHYS A, V118, P1151 JAGDALE GB, 1997, J THERM BIOL, V22, P245 JAGDALE GB, 1998, FUND APPL NEMATOL, V21, P177 JAGDALE GB, 1998, FUNDAM APPL NEMATOL, V20, P147 KAYA HK, 1990, ENTOMOPATHOGENIC NEM, P93 LAWRENCE JV, 1977, APPL ENVIRON MICROB, V33, P482 LEHNINGER AI, 1979, BIOCHEMISTRY LIN JJ, 1995, PHYSIOL ZOOL, V68, P114 MARCUS NH, 1977, COMP BIOCH PHYSL, V58, P109 NEALSON KH, 1990, ENTOMOPATHOGENIC NEM, P271 RATKOWSKY DA, 1982, J BACTERIOL, V149, P1 SMART GC, 1995, J NEMATOL S, V27, P529 SOKAL RR, 1995, BIOMETRY PRINCIPLES, P242 SUMNER JL, 1969, J GEN MICROBIOL, V59, P215 SUUTARI M, 1994, CRIT REV MICROBIOL, V20, P285 YAMAWAKI H, 1979, COMP BIOCH PHYSL B, V62, P89; NR: 36; TC: 0; J9: CAN J MICROBIOL; PG: 10; GA: 430QZ
Source type: Electronic(1)
Language
- English
Subjects
- Immunology
- Xenorhabdus
- THERMAL ADAPTATION
- isozyme
- mesophile
- PHOTORHABDUS
- Steinernematidae
- INSECT PATHOGENIC NEMATODES
- Enterobacteriaceae
- lipids
- TEMPERATURE
- fatty acid
- DEHYDROGENASE
- Biotechnology & Applied Microbiology
- Microbiology
- Biochemistry & Molecular Biology
- psychrotroph
- LUMINESCENS