Genre
- Journal Article
Previous work has shown that it is possible to image whole uncoated chloroplasts using scanning tunneling microscopy (STM), provided this is done in solution using tunnelling currents below about 100 pA. More recent images include some which suggest that the STM is sensitive to dynamic processes occurring on chloroplast surfaces. Current-versus-distance curves and dI/ds measurements are consistent with tunnelling between the tip and sample surface, and relatively small deformations of the sample surface due to tip-sample forces. Attempts to use the same imaging conditions on bacteria were unsuccessful.
UNIV PRINCE EDWARD ISL,DEPT BIOL,CHARLOTTETOWN,PE C1A 4P3,CANADA.; DAHN, DC, UNIV PRINCE EDWARD ISL,DEPT PHYS,CHARLOTTETOWN,PE C1A 4P3,CANADA.
CHICAGO; PO BOX 66507, AMF O'HARE, CHICAGO, IL 60666
SCANNING MICROSCOPY INT
PT: J; CR: COOMBS JH, 1986, IBM J RES DEV, V30, P455 DAHN DC, 1992, ULTRAMICROSCOPY, V42, P1222 DAI JW, 1991, J VAC SCI TECHNOL B, V9, P1184 DOWLING TE, 1990, MOL SYSTEMATICS, P250 GARCIA AA, 1993, SCANNING MICROSCOPY, V7, P577 GUCKENBERGER R, 1989, ULTRAMICROSCOPY, V31, P327 HALL BK, 1984, CRANIOFACIAL MESENCH, P1 HORBER JKH, 1992, SCANNING MICROSCOPY, V6, P919 ITO E, 1991, BIOCHEM BIOPH RES CO, V177, P636 MAINSBRIDGE B, 1991, J VAC SCI TECHNOL B, V9, P1259 MAMIN HJ, 1986, PHYS REV B, V34, P9015 NAGAHARA LA, 1989, REV SCI INSTRUM, V60, P3128 PARK RB, 1964, SCIENCE, V144, P1009 ROCK CD, 1992, PLANT PHYSIOL, V100, P1796 RUPPERSBERG JP, 1989, FEBS LETT, V257, P460 SASS JK, 1991, J PHYS-CONDENS MAT, V3, S121 SCHNELL DJ, 1990, J CELL BIOL, V111, P1825 TANG SL, 1993, PHYS REV B, V47, P3850 TRIBE M, 1972, CHLOROPLASTS MITOCHO, P24 WEIER TE, 1965, J ULTRASTRUCT RES, V13, P92; NR: 20; TC: 1; J9: SCANNING MICROSCOPY; PG: 6; GA: TB725
Source type: Electronic(1)
Language
- English
Subjects
- PROTEIN
- CHLOROPLAST ENVELOPE MEMBRANE
- BIOLOGICAL APPLICATIONS OF STM
- SCANNING TUNNELING MICROSCOPY (STM)
- STM
- CHLOROPLAST
- MEMBRANE
- Microscopy
- STM IN LIQUIDS