Genre
- Journal Article
The matrix metalloproteinases (MMPs) are thought to play key roles in tumour formation and malignant progression. The present study demonstrates alterations in the regulation of matrix metalloproteinase-2 (MMP-2) expression in response to the phorbol ester tumour promoter, PMA, in a H-ras transformed cell line, NR3, which is capable of benign tumour formation. PMA treatment of NR3 cells resulted in decreased expression of MMP-2 mRNA levels. Following a lag period, an accompanying change in gelatinolytic activity was also found. These PMA-mediated alterations in MMP-2 mRNA levels were independent of de novo protein synthesis and involved both transcriptional and post-transcriptional events. Most notably, PMA regulates MMP-2 mRNA expression through a mechanism involving message de-stabilization. Additionally, protein kinase C mediated events were found to play a role(s) in the regulation of MMP-2 message expression in NR3 cells. This study demonstrates several novel aspects regarding the regulation of MMP-2 expression in a H-ras transformed cell line and thereby provides further insight into the altered growth regulatory programs associated with H-ras mediated cellular transformation.
St Michaels Hosp, Dept Lab Med & Pathobiol, Toronto, ON M5B 1A6, Canada. Univ Toronto, Toronto, ON, Canada.; Hurta, RAR, St Michaels Hosp, Dept Lab Med & Pathobiol, 1-001 E Annex,38 Shuter St, Toronto, ON M5B 1A6, Canada.
DORDRECHT; SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS
KLUWER ACADEMIC PUBL
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Source type: Electronic(1)
Language
- English
Subjects
- FACTOR-BETA(1)
- FIBROBLAST
- tumour promoter
- PROTEIN KINASE-C
- DECARBOXYLASE GENE-EXPRESSION
- MMP-2
- RIBONUCLEOTIDE REDUCTASE
- GROWTH-FACTOR
- GELATINASE-A
- Cell Biology
- ornithine decarboxylase
- regulation
- INTERSTITIAL
- COLLAGENASE
- altered expression
- IV COLLAGENASE
- TISSUE INHIBITOR