Genre
- Journal Article
Intestinal eosinophilic granule cells (EGCs) of the rainbow trout (Oncorhynchus mykiss) have been likened to mammalian mast cells and degranulate in response to stimulation by capsaicin and substance P. This investigation was conducted to examine the effects of capsaicin and substance P on the trout intestinal EGC population and to quantify the different morphologies following systemic administration. Rainbow trout were injected intraperitoneally with capsaicin, substance P, serotonin, or vehicle controls (0.5 mug/g body weight). Fish were killed at timed intervals and the mid intestine was processed for light and electron microscopy. The number of EGCs which could be observed in the stratum compactum was quantified for each treatment over the time course of the experiment. EGCs could be classified ultrastructurally into 1 of 5 categories based on their granule morphology. The cell frequencies and relative proportion of each cell class were analyzed statistically. The frequency of EGCs in the stratum compactum of fish injected with capsaicin or substance P significantly decreased post-injection (P < 0.05) compared to controls (saline- and BSA-injected fish). Serotonin had no effect on EGC frequency, morphology, or distribution as compared with saline. Stimulation with capsaicin and substance P resulted in time-dependent changes in both EGC granule morphology and distribution within the intestinal mucosa. Following an apparent migration of EGCs to the lamina propria and degranulation, small granuled EGC-like cells appeared first in the lamina propria and then later in the stratum compactum. The significance of the stratum compactum as a depot for intestinal EGCs and the site for EGC maturation is discussed.
POWELL, MD, UNIV PRINCE EDWARD ISL,ATLANTIC VET COLL,DEPT ANAT & PHYSIOL,CHARLOTTETOWN C1A 4P3,PEI,CANADA.
NEW YORK; DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012
WILEY-LISS
PT: J; CR: ANDERSON C, 1983, CELL TISSUE RES, V230, P377 ANDERSON CR, 1990, CELL TISSUE RES, V259, P379 AVTALION RR, 1980, PHYLOGENY IMMUNOLOGI, P113 BEFUS AD, 1982, J IMMUNOL, V128, P2475 BERGERON T, 1982, THESIS U GUELPH CANA BERGERON T, 1983, CAN J ZOOL, V61, P133 BIENENSTOCK J, 1987, INT ARCH ALLER A IMM, V82, P238 BIENENSTOCK J, 1989, MAST CELL BASOPHIL D, P275 BJENNING C, 1988, HISTOCHEMISTRY, V88, P155 BUCK SH, 1986, PHARMACOL REV, V38, P179 DAVIDSON S, 1980, IMMUNOLOGY, V48, P439 ELLIS AE, 1977, J FISH BIOL, V11, P453 ELLIS AE, 1985, DEV COMP IMMUNOL, V9, P251 ELLIS AE, 1988, FISH VACCINATION, P32 EZEASOR DN, 1980, J FISH BIOL, V17, P619 FINN JP, 1971, J FISH BIOL, V3, P463 GLANTZ SA, 1987, PRIMER BIOSTATISTICS GULLAND GL, 1898, ANAT ANZEIGER, V14, P441 HODGINS HO, 1967, J IMMUNOL, V99, P534 HOLMGREN S, 1985, NEUROSCIENCE, V14, P683 IRANI AMA, 1989, CLIN EXP ALLERGY, V19, P143 JAMUR MC, 1988, CELL MOL BIOL, V34, P231 JOHNSON AR, 1973, P SOC EXP BIOL MED, V142, P1252 KITAZAWA T, 1989, BRIT J PHARMACOL, V98, P781 LAMAS J, 1991, FISH SHELLFISH IMMUN, V1, P187 MARZELLA L, 1987, LYSOSOMES THEIR ROLE, P319 MOUSLI M, 1989, J PHARMACOL EXP THER, V250, P329 NILSSON G, 1989, THESIS UPPSALA NILSSON G, 1990, CELL TISSUE RES, V262, P125 NOVIKOFF AB, 1976, CELL ORGANELLES, P27 PERNOW B, 1983, PHARMACOL REV, V35, P85 POWELL MD, 1990, J FISH BIOL, V37, P495 POWELL MD, 1991, CELL TISSUE RES, V266, P469 POWELL MD, 1992, AQUACULTURE, V100, P328 POWELL MD, 1992, HISTOL HISTOPATHOL, V7, P301 REIMSCHUESSEL R, 1987, J FISH DIS, V10, P319 ROBERTS RJ, 1971, J FISH BIOL, V4, P87 SHANAHAN F, 1985, J IMMUNOL, V135, P1331 SHARP GJE, 1989, J FISH BIOL, V35, P781 SMITH HE, 1975, THESIS U STIRLING ST STEAD RH, 1987, P NATL ACAD SCI USA, V84, P2975 STEAD RH, 1989, GASTROENTEROLOGY, V97, P575 SWEENEY MI, 1989, J PHARMACOL EXP THER, V248, P447 VALLEJO AN, 1989, DEV COMP IMMUNOL, V13, P133 WATSON AHD, 1979, CELL TISSUE RES, V197, P155 YASUTAKE WT, 1983, 150 US DEP INT FISH; NR: 46; TC: 10; J9: J EXP ZOOL; PG: 12; GA: LC103
Source type: Electronic(1)
Language
- English
Subjects
- fish
- SALMO-GAIRDNERI RICHARDSON
- MUCOSAL MAST-CELLS
- NEURONS
- 5-HYDROXYTRYPTAMINE
- NERVES
- zoology
- GUT
- ULTRASTRUCTURE
- Histamine
- RELEASE