Genre
- Journal Article
A total of 20 hybridoma cell lines secreting monoclonal antibodies (MAbs) against E. coli expressed bovine herpesvirus-1 (BHV-1) gD fusion protein were produced following the fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized previously with immunoaffinity purified BHV-1 gD fusion protein. An indirect fluorescent antibody test (IFAT) using BHV-1 infected MDBK cells was used for the selection of positive hybridomas secreting specific antibody. The monoclonal antibody isotypes were 11 IgM, six IgG2b, one IgG1 and two IgG3. All MAbs reacted positively with the E. coli expressed BHV-1 gD fusion protein, BHV-1 infected MDBK cell lysates and PCR BHV-1 gD transcription-translation polypeptide antigens by an ELISA.
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
NETHERLANDS
LR: 20061115; PUBM: Print; JID: 8005839; 0 (Antibodies, Monoclonal); 0 (Antigens, Viral); 0 (DNA Primers); 0 (Immunoglobulin Isotypes); 0 (Recombinant Fusion Proteins); 0 (Viral Proteins); 0 (bovine herpesvirus type-1 glycoproteins); ppublish
Source type: Electronic(1)
Language
- English
Subjects
- gene expression
- Herpesvirus 1, Bovine/immunology
- Antigens, Viral/genetics
- Evaluation Studies
- Viral Proteins/genetics/immunology
- Hybridomas/immunology
- Cell Line
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli/genetics
- DNA Primers/genetics
- animals
- cattle
- Immunoglobulin Isotypes/immunology
- Antibodies, Monoclonal/immunology
- Fluorescent Antibody Technique, Indirect
- Mice
- Virology/methods
- Base Sequence
- Recombinant Fusion Proteins/genetics/immunology