Use of bulk tank and milk filter cultures in screening in Streptococcus agalactiae and coagulase-positive staphylococci

The use of a single tank milk culture and a single milk filter culture was studied for their suitability as screening tests for coagulase-positive staphylococci and Streptococcus agalactiae. Bulk tank and bulk tank milk filter cultures were compared with quarter milk cultures taken from individual cows at 49 Ohio, USA, dairy herds selected from all Ohio dairy herds by a stratified random sampling scheme. Individual cow quarter milk samples were collected from a sample of all milking cows using a sampling scheme designed to detect an organism present in 2% of quarters, with 95% confidence intervals between 1 and 3%. 17 (35%) herds had one or more cows positive for S. agalactiae and 34 (69%) had one or more cows positive for coagulase-positive staphylococci. Using the results of individual cow sampling as the standard, the sensitivity for S. agalactiae was estimated as 23.5% for a single milk filter sample and 35.3% for a single bulk tank milk sample. The sensitivity for coagulase-positive staphylococci was estimated as 52.9% for a single milk filter culture and 41.2% for a single bulk tank milk culture. Based on these results and those of others, it appears that a single bulk tank or milk filter sample has a relatively low sensitivity for both coagulase-positive staphylococci and S. agalactiae, making these poor screening tests for the presence of these pathogens within a dairy herd..